Ms. Laura J. Itle Profile

Laura J. Itle

at Pennsylvania State Univ

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Proceedings Article | 7 December 2004 Paper

Cell-based bioassays in microfluidic systems

Laura Itle, Jeanna Zguris, Michael Pishko

Proceedings Volume 5588, (2004) https://doi.org/10.1117/12.569094

KEYWORDS: Microfluidics, Optical lithography, Chemical elements, Chemical engineering, Sensors, Glasses, Photomasks, Sensing systems, Luminescence, Polymers

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The development of cell-based bioassays for high throughput drug screening or the sensing of biotoxins is contingent on the development of whole cell sensors for specific changes in intracellular conditions and the integration of those systems into sample delivery devices. Here we show the feasibility of using a 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate, a fluorescent dye capable of responding to changes in intracellular pH, as a detection method for the bacterial endotoxin, lipopolysaccharide. We used photolithography to entrap cells with this dye within poly(ethylene) glyocol diacrylate hydrogels in microfluidic channels. After 18 hours of exposure to lipopolysaccharide, we were able to see visible changes in the fluorescent pattern. This work shows the feasibility of using whole cell based biosensors within microfluidic networks to detect cellular changes in response to exogenous agents.

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