Dr. Lindsey M. Brier Profile

Dr. Lindsey M. Brier

at Washington University in St. Louis

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Publications (6)

SPIE Journal Paper | 28 November 2024 Open Access

Zika virus encephalitis causes transient reduction of functional cortical connectivity

Shannon Agner, Lindsey Brier, Jeremy Hill, Ethan Liu, Annie Bice, Rachel Rahn, Shengxuan Chen, Joseph Culver, Robyn Klein

NPh, Vol. 12, Issue S1, S14603, (November 2024) https://doi.org/10.1117/12.10.1117/1.NPh.12.S1.S14603

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SignificanceDetermining the long-term cognitive impact of infections is clinically challenging. Using functional cortical connectivity, we demonstrate that interhemispheric cortical connectivity is decreased in individuals with acute Zika virus (ZIKV) encephalitis. This correlates with decreased presynaptic terminals in the somatosensory cortex. During recovery from ZIKV infection, presynaptic terminals recover, which is associated with recovered interhemispheric connectivity. This supports the contribution of synapses in the cortex to functional networks in the brain, which can be detected by widefield optical imaging. Although myeloid cell and astrocyte numbers are still increased during recovery, RNA transcription of multiple proinflammatory cytokines that increase during acute infection decreases to levels comparable to mock-infected mice during recovery. These findings also suggest that the immune response and cytokine-mediated neuroinflammation play significant roles in the integrity of brain networks during and after viral encephalitis.AimWe hypothesized that widefield optical imaging would allow us to assess functional cortical network disruption by ZIKV, including hippocampal-cortical networks.ApproachWe use widefield optical imaging to measure cortical functional connectivity (FC) in mice during acute infection with, and recovery from, intracranial infection with a mouse-adapted strain of ZIKV.ResultsAcute ZIKV infection leads to high levels of myeloid cell activation, with loss of neurons and presynaptic termini in the cerebral cortex and associated loss of FC primarily within the somatosensory cortex. During recovery, neuron numbers, synapses, and FC recover to levels near those of healthy mice. However, hippocampal injury and impaired spatial cognition persist. The magnitude of activated myeloid cells during acute infection predicted both recovery of synapses and the degree of FC recovery after recovery from ZIKV infection.ConclusionsThese findings suggest that a robust inflammatory response may contribute to the health of functional brain networks after recovery from infection.

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SPIE Journal Paper | 1 March 2023 Open Access

Open-source statistical and data processing tools for wide-field optical imaging data in mice

Lindsey M. Brier, Joseph P. Culver

NPh, Vol. 10, Issue 01, 016601, (March 2023) https://doi.org/10.1117/12.10.1117/1.NPh.10.1.016601

KEYWORDS: Data processing, Functional magnetic resonance imaging, Statistical analysis, Optical imaging, Visualization, Calcium, Neurophotonics, MATLAB, Data modeling, Brain

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Proceedings Article | 28 April 2022 Poster + Presentation

Simultaneous functional and thermal imaging of mouse cortex

Hunter Banks, Shengxuan Chen, Jonathan Bumstead, Lindsey Brier, Annie Bice, Seana Gaines, Joseph Culver

Proceedings Volume PC11946, PC119460W (2022) https://doi.org/10.1117/12.2608942

KEYWORDS: Thermography, Signal to noise ratio, Brain, Neuroimaging, Infrared radiation, Infrared imaging, Two photon imaging, Two photon excitation microscopy, Optical design, Laser safety

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Proceedings Article | 5 March 2021 Presentation

Photothermal effects and functional neuroimaging using large field two-photon microscopy in mice

Hunter Banks, Jon Bumstead, Lindsey Brier, Annie Bice, Seana Gaines, Joseph Culver

Proceedings Volume 11629, 116291X (2021) https://doi.org/10.1117/12.2578578

KEYWORDS: Neuroimaging, Photothermal effect, Two photon excitation microscopy, Imaging systems, Brain, Temporal resolution, Neurons, Motion analysis, Microscopes, Mechanical engineering

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Proceedings Article | 9 March 2020 Presentation

Wide-field multi-scale areal parcellation of neural circuits in mice (Conference Presentation)

Lindsey Brier, Jonathan Bumstead, Hunter Banks, Annie Bice, Joseph Culver

Proceedings Volume 11226, 1122619 (2020) https://doi.org/10.1117/12.2546317

KEYWORDS: Calcium, Brain, Light emitting diodes, Sensors, Neural networks, Magnetic resonance imaging, Luminescence, Brain mapping, Electron multiplying charge coupled devices, Hemodynamics

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SPIE Journal Paper | 13 July 2019 Open Access

Separability of calcium slow waves and functional connectivity during wake, sleep, and anesthesia

Lindsey Brier, Eric Landsness, Abraham Snyder, Patrick Wright, Grant Baxter, Adam Bauer, Jin-Moo Lee, Joseph Culver

NPh, Vol. 6, Issue 03, 035002, (July 2019) https://doi.org/10.1117/12.10.1117/1.NPh.6.3.035002

KEYWORDS: Brain, Calcium, Electroencephalography, Brain mapping, Principal component analysis, Binary data, Video, Modulation, Luminescence, Neurophotonics

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