Mueller matrix polarimetry along with polar decomposition algorithm was employed for the characterization of ex vivo normal and adenocarcinoma human colon tissues by polarized light in the visible spectral range (425–725 nm). Six derived polarization metrics [total diattenuation (DT), retardance (RT), depolarization (ΔT), linear diattenuation (DL), retardance (δ), and depolarization (ΔL)] were compared for normal and adenocarcinoma colon tissue samples. The results show that all six polarimetric properties for adenocarcinoma samples were significantly higher as compared to the normal samples for all wavelengths. The Wilcoxon rank sum test illustrated that total retardance is a good candidate for the discrimination of normal and adenocarcinoma colon samples. Support vector machine classification for normal and adenocarcinoma based on the four polarization properties spectra (ΔT, ΔL, RT,and δ) yielded 100% accuracy, sensitivity, and specificity, while both DT and DL showed 66.6%, 33.3%, and 83.3% accuracy, sensitivity, and specificity, respectively. The combination of polarization analysis and given classification methods provides a framework to distinguish the normal and cancerous tissues.
We demonstrate significant differences in the propagation of polarized laser light through biological tissue
phantom. The Stokes vectors along with degree of linearly and circularly polarized light were measured with
stokes polarimetry techniques. The measurements were performed on dense and diluted tissue phantoms that
consisted of soybean oil interloped. Liquid crystal variable retarder (LCVR) Stokes polarimeter is used for
either rotating the major axis of elliptically polarized light or for converting an input linearly polarized beam
into an arbitrary elliptically polarized beam. This system makes possible a direct measurement of a
component of the Stokes vector with phase change detection of polarization modulation for polarimetric
measurements of turbid media and biological tissue.
The polarization sensitive optical coherence tomography (OCT) system provides useful informations about the biological tissues. The exact tissue parameters measurement and comparison predicts about the malignant and normal tissues. The degree of polarization changes with the depth of tissue samples. We have established the analytical modeling with Jones-Mueller matrix for imaging technique, which experimentally extract the birefringence, depolarization, absorption and scattering information of tissues. The Jones matrix of thermally treated porcine tendon showed a reduction of birefringence from thermal damage. The Jones matrices of porcine skin and bovine cartilage also revealed that the density and orientation of the collagen fibers in porcine skin and bovine cartilage are not distributed as uniformly as in porcine tendon. Birefringence is sensitive to changes in tissue because it is based on phase contrast.
Laser transmittion and scattering technique, including depolarization of wave applied to biological particles provide a sample way for diagnostic and treatment of skin lesion and breast cancer. Laser polarization imaging system is described for non invasive and non radioactive detection. The system described in this paper generates 16 full out put Mueller matrix for characterization of turbid medium. In this work we describe the scattering and depolarization of electromagnetic radiation through biological turbid medium. This research work provides a base work for designing quick model of polarized laser tissues imaging.
The polarization sensitive optical coherence tomography (OCT) system provides useful informations about the biological tissues. The exact tissue parameters measurement and comparison predicts about the malignant and normal tissues. The degree of polarization changes with the depth of tissue samples. We have established the analytical modeling with Jones-Mueller matrix for imaging technique, which experimentally extract the birefringence, depolarization, absorption and scattering information of tissues. The Jones matrix of thermally treated porcine tendon showed a reduction of birefringence from thermal damage. The Jones matrices of porcine skin and bovine cartilage also revealed that the density and orientation of the collagen fibers in porcine skin and bovine cartilage are not distributed as uniformly as in porcine tendon. Birefringence is sensitive to changes in tissue because it is based on phase contrast.
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