Microstructured optical fibers (MOF) are a highly efficient support for the design and construction of sensitive and lowcost sensors. Currently, molecular imprinting is one of the promising method of MOF inner surface functionalization. Molecularly imprinted polymers (MIP) can be synthesized at MOF inner surface and intensify the selectivity and efficiency of target molecules sorption. This approach can be the base for selective sorption and detection of target molecules on a single sensor device. This paper describes MOF modification by MIP selective for bovine serum albumin (BSA). Polyaniline was used as matrix MIP polymer and was obtained by chemical oxidate polymerization of aniline in soft acid conditions. MOF transmission spectrum were used for monitoring of bonding between the protein molecules and MIP.
Optical sensing is a powerful tool for recognition interactions in biosystems. Nowadays sensor systems allow to determinate the biomolecules interactions in biological fluids with high sensitivity, reproducibility and minimum standard error. Usually analyses are carried out in a heterogeneous format, which significantly complicates the procedure and increases the single analysis cost. The swap from heterogeneous to homogeneous analysis format is a promising solution. Microstructured optical fibers (MOFs) are a possible platform for creation of such sensors. Manuscript describe the possibility to detect antigen-antibody interactions in the hollow core microstructured optical fibers (HC MOFs). The determination of such interactions was carried out both in heterogeneous and homogeneous formats. Results demonstrate the prospects of HC MOF as a platform for the detection of antigen-antibody interactions.
Alloyed quantum dots (QDs) are the popular material for the fabrication of the light-emitting diodes because of their optical parameters advantages. One of the most interesting features peculiar to this QDs class is the ability to controlled size-independent emission peak tuning via compound amounts changing. The same advantages can be valuable in bioanalytical methods and sensor systems development. Facile one-pot synthesis of the bright quaternary alloyed QDs made by CdxZn1-xSeyS1-y/ZnS semiconductor composition with narrow (FWHM < 26 nm) emission peak (548 nm) and photoluminescence quantum yield (PLQY) up to 50% was described. Obtained QDs were stabilized in water by the dihydrolipoic acid (DHLA) ligand exchange approach with insignificant PLQY loss relative initial (16%) and great colloidal stability period (6 months). This article summarizes the easiest ways of the alloyed QDs synthesis and subsequent hydrophilization for the potential simplification of their large-amounts manufacturing procedure.
In this article, we report the effect of the pH of bovine serum albumin and egg serum albumin solutions on the microstructured optical fiber transmission spectra fluctuations near proteins isoelectric point. The demonstrated effect may be important in the development of perspective optical microstructured optical fiber-based biosensors in a labeled and label-free formats for a wide range of analytes.
One of the most interesting modern platforms for fiber-optic biosensor are microstructured optical fibers. The analytical signal in label-free analysis can be determined by the following mechanisms: changes in the thickness of biomolecule layers immobilized on the surface of the fiber hollow fiber core; small changes in the refractive index of the analyzed solution in the fiber hollow core of the fiber and the absorption spectrum of the analyzed solution. In this article, we report data on the effect of different salt solutions small concentration changes on the MOF HC transmission spectra
Microstructural optical waveguides with the hollow core are actively studied as a promising support for heterogeneous immunoassay in development of new optical biosensor elements for medicine and biology. Overcoming of the limitations associated with the low sorption capacity of glass used for the waveguides production is a crucial step for this assay format. In this work the possibility of silanization of microstructural optical waveguides with the hollow core using (3-glycidyloxypropyl) trimethoxysilane and their further application to enzymatic immunoassay was studied.
The use of photonic crystal fibers as a basis elements for biosensor construction is a perspective trend. The advantages of this approach are the use of micro and nano volumes of samples and a significant strengthening of the analytical signal while increasing the optical path length. Quantum dots are the most promising fluorescent markers for use in the photonic crystal fibers based analysis.
The advantages of quantum dots, in this case, are associated with stability, wide range of excitation and extremely narrow range of high luminescence intensity. In this work we have investigated the behavior and optical properties of CdSe core-shell nanocrystals (quantum dots) after they including into hollow central defect of hollow core chirped photonic crystal fibers with internal surface modified by polyaniline films.
Photonic crystal fibers (PCFs) are one of the most promising materials for biosensors construction due to their unique optical properties. The modification of PCF by noble metal nanoparticles (NPs) provides the SPR and SERS signal detection where as the application amino group-containing compounds allows efficient binding of biomolecules. In this work the internal surface of glass hollow core photonic crystal fibers (HC-PCFs) has been modified Ag and Au nanoparticles using three different approaches. PCFs were treated by: 1) mixture of NPs and precursors for silanization (tetraethoxysilane (TEOS) and (3-aminopropyl)triethoxysilane (APTES)); 2) alternately deposition of polyelectrolytes and NPs, 3) mixture of chitosan with NPs. The shift of local maxima in the HC-PCF transmission spectrum has been selected as a signal for estimating the amount of NPs on the HC-PCF inner surface. The most efficient techniques were the chitosan application for Ag NPs and silanization for Au NPs. The obtaining PCFs could be useful for creating biosensitive elements.
Photonic crystal fibers (PCFs) with a hollow core are one of the most promising solid support of fiber-optic sensors. The main advantages of PCF as sensor elements in clinical analysis are minimization of optical interactions from the sample and the ability to analyze small volume of samples. At the same time, low sorption capacity of glass which is the basic material for the fabrication of the PCF, limits their use in the development of biosensors. Modification of the inner surface of the PCF can be the solution of the problem.
In this work the synthesis of self-assembled films of polyaniline (PANI) on the inner surface of the PCFs was carried out. The modified PCFs were studied by spectroscopy and electron microscopy. It was found that the covering of the inner surface of the PCFs with PANI leads to a shift of the local maximums of the transmission spectrum PCFs up to 25 nm. These makes possible to design the method of varying of photonic bandgaps location.
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