KEYWORDS: Visualization, Optical coherence tomography, Retina, 3D image processing, Speckle, Data acquisition, Calibration, In vivo imaging, Interferometers, Human-machine interfaces
We demonstrate an intensity-based motion sensitive method, called differential logarithmic intensity variance
(DLOGIV), for 3D microvasculature imaging and foveal avascular zone (FAZ) visualization in the in vivo human retina
using swept source optical coherence tomography (SS-OCT) at 1060 nm. A motion sensitive SS-OCT system was
developed operating at 50,000 A-lines/s with 5.9 μm axial resolution, and used to collect 3D images over 4 mm2 in a
normal subject eye. Multiple B-scans were acquired at each individual slice through the retina and the variance of
differences of logarithmic intensities as well as the differential phase variances (DPV) was calculated to identify regions
of motion (microvasculature). En face DLOGIV image were capable of capturing the microvasculature through depth
with an equal performance compared to the DPV.
KEYWORDS: Optical coherence tomography, Visualization, Plasma display panels, Retina, In vivo imaging, Capillaries, Phase measurement, Data acquisition, Imaging systems, Signal to noise ratio
Human retinal and choroidal vasculature was visualized by a differential phase-contrast (DPC) method using high-speed, swept-source optical coherence tomography (SS-OCT) at 1060 nm. The vasculature was recognized as regions of motion by creating differential phase-variance (DPV) tomograms: multiple B-scans of individual slices through the retina were collected and the variance of the phase differences was calculated. DPV captured the small vessels and the meshwork of capillaries associated with the inner retina in en-face images over 4 mm2. The swept-source laser at 1060 nm offered the needed phase sensitivity to perform DPV and generated en-face images that capture motion in the inner choroidal layer exceeding the capabilities of previous spectrometer-based instruments. In comparison with the power Doppler phase-shift method, DPV provided better visualization of the foveal avascular zone in en-face images.
A differential phase contrast (DPC) method is validated for in vivo human retinal and choroidal vasculature visualization
using high-speed swept-source optical coherence tomography (SS-OCT) at 1060 nm. The vasculature was identified as
regions of motion by creating differential phase variance (DPV) tomograms: multiple B-scans were collected of
individual slices through the retina and the variance of the phase differences was calculated. DPV captured the small
vessels and the meshwork of capillaries associated with the inner retina in en face images over 4 mm2 in a normal
subject. En face DPV images were capable of capturing the microvasculature and regions of motion through the inner
retina and choroid.
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