A shortened approach to measure mitochondrial capacity using Near-Infrared Spectroscopy (NIRS) was evaluated. Comparisons were made between 6-occlusion versus 22-occlusion protocols in two data sets (bicep, forearm). A third data set evaluated four serial 6-occlusion tests. The rate constants were not different between 22-cuff and 6-cuff for bicep (p=0.56) and forearm (p=0.76). The correlations were R2=0.9 for the bicep and R2=0.93 for the forearm. Mitochondrial capacity was not different between the four tests (P>0.05). The 6-Cuff analysis provided the same results in less time. There were no order effects for the rate constants for repeated 6-cuff tests of mitochondrial capacity.
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