Laser technique has been applied to measure cyclic contractile movement of cultured myotubes in vitro. The designed
measurement system includes light source (helium neon, 632.8 nm wave length), charge-coupled devise cameras and
detectors. Cyclic contraction of myotubes cultured from C2C12 (mouse myoblast) was generated by cyclic electric
pulses (amplitude < 60 V, 1 ms width) through electrodes of platinum wire dipped in the medium. The spectrum of
fluctuating intensity of the transmitted laser beam through the myotubes, which periodically repeated contraction and
relaxation, was analyzed. The results show that the designed laser system is effective to detect frequency of cyclic
contractile movement of myotubes between 0.5 Hz and 5 Hz.
The cyclic micro-deformation of the arterial wall with pulsatile flow was measured to get fundamental data for estimation of the mechanical stress in endothelial cells. The descending aorta (1-2 mm diameter) of an anesthetized rat was exposed under thoracotomy. The displacement measuring system was assembled with the charge coupled devise (CCD) of laser sensor. The movement of laser beam (670 nm wave length) reflected at the vessel wall was calibrated to the movement of the arterial wall. The fluctuating movement was also measured at four points marked on the vessel wall with CCD camera to distinguish the circumferential movement from the longitudinal one. The results showed that the present designed system has enough resolution to measure the arterial vessel wall cyclic-micro-fluctuation, which is 10 percent of diameter in the circumferential direction without deformation in the longitudinal direction with the cardiac beating in vivo.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.