Pleomorphic invasive lobular carcinoma (pILC) is a distinct morphological variant of invasive lobular carcinoma (ILC), which is more aggressive than classic invasive lobular carcinoma (cILC) with worse overall prognosis. It is critical to distinguish between pILC and cILC for management and treatment of patients. Multiphoton microscopy (MPM),based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), produces subcellular resolution images to show detailed information on structure and cell morphology of tissue sections without staining. In this work, MPM was introduced for label-free identification of normal breast lobules, pILC and cILC, which is consistent with haematoxylin and eosin (H&E) staining. Furthermore, analysis of nuclear area is used to quantitatively reflect their cytological characteristic. These results demonstrate that, MPM has the ability to distinguish between normal breast lobules, cILC, and pILC by histopathological features, which can provide additional indicators to determine adequate treatment strategies of patients with pILC for pathologists and surgeons. In the future, MPM may be used as a diagnostic tool of lobular cancer into routine clinical use.
Macrophages are at the center of the invasion and play a protumoral role in the tumor microenvironment. They can facilitate angiogenesis, extracellular matrix remodeling, invasion and migration of cancer cells, while inhibit the antitumoral immune surveillance. Tumor-associated macrophages are a major component of malignant tumors, which can stimulate the progress of tumors. In many human cancers, the presence of macrophages in tumor microenvironment has been correlated with unfavorable prognosis. Therefore, visual diagnosis of macrophages in tumor microenvironment is great significance for us to comprehend the occurrence and progress of tumor. Multiphoton microscopy (MPM) with subcellular resolution based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) is very suitable for real-time detecting morphological and structural changes in biological tissues without tissue staining and exogenous probe molecule. In this study, we describe the use of label-free MPM for analyzing the intratumoral distribution and morphological changes of macrophages in breast tumor microenvironment. Our results indicated that MPM can accurately identify macrophages in breast tumor tissues. MPM images were well consistent hematoxylin and eosin (H&E) stained images. This work would provide the basis for further quantifying these changes using MPM. With the development of miniaturized imaging devices, MPM may be a promising imaging technique for clinicians to study various structural features in tumor microenvironment.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.