Dr. Stéphane Bancelin Profile

Dr. Stéphane Bancelin

at Univ de Bordeaux

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Publications (6)

SPIE Journal Paper | 27 March 2024 Open Access

Understanding the nervous system: lessons from Frontiers in Neurophotonics

Yves De Koninck, Johanna Alonso, Stéphane Bancelin, Jean-Claude Béïque, Erik Bélanger, Catherine Bouchard, Marco Canossa, Johan Chaniot, Daniel Choquet, Marie-Ève Crochetière, Nanke Cui, Lydia Danglot, Paul De Koninck, Anna Devor, Mathieu Ducros, Angela Getz, Mohamed Haouat, Iván Coto Hernández, Nate Jowett, Iason Keramidis, Céline Larivière-Loiselle, Flavie Lavoie-Cardinal, Harold MacGillavry, Asiye Malkoç, Mattia Mancinelli, Pierre Marquet, Steven Minderler, Maxime Moreaud, U. Valentin Nägerl, Katerina Papanikolopoulou, Marie-Eve Paquet, Lorenzo Pavesi, David Perrais, Romain Sansonetti, Martin Thunemann, Beatrice Vignoli, Jenny Yau, Clara Zaccaria

NPh, Vol. 11, Issue 01, 014415, (March 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.1.014415

KEYWORDS: Biological imaging, Neurophotonics, Proteins, Nervous system, In vivo imaging, Neurons, Brain, Optogenetics, Electrodes, Neuroimaging

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SPIE Journal Paper | 17 May 2023 Open Access

Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy

Stéphane Bancelin, Luc Mercier, Johannes Roos, Mohamed Belkadi, Thomas Pfeiffer, Sun Kwang Kim, U. Valentin Nägerl

NPh, Vol. 10, Issue 04, 044402, (May 2023) https://doi.org/10.1117/12.10.1117/1.NPh.10.4.044402

KEYWORDS: Stimulated emission depletion microscopy, Windows, Spine, Point spread functions, Cranial windows, Spatial resolution, Brain, Microscopy, In vivo imaging, Objectives

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SPIE Journal Paper | 14 June 2021 Open Access

Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue

Stéphane Bancelin, Luc Mercier, Emanuele Murana, U. Valentin Nägerl

NPh, Vol. 8, Issue 03, 035001, (June 2021) https://doi.org/10.1117/12.10.1117/1.NPh.8.3.035001

KEYWORDS: Stimulated emission depletion microscopy, Monochromatic aberrations, Brain, Aberration correction, Tissues, Neuroimaging, Spine, Microscopy, Spatial light modulators, Gold

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Proceedings Article | 1 April 2020 Presentation

Ex-vivo multiscale biomechanics in murine skin and human cornea using multiphoton microscopy (Conference Presentation)

Stéphane Bancelin, Barbara Lynch, Guillaume Ducourthial, Christelle Bonod-Bidaud, Florence Ruggiero, Aurélie Benoit, Gaël Latour, Jean-Marc Allain, Marie-Claire Schanne-Klein

Proceedings Volume 11362, 1136204 (2020) https://doi.org/10.1117/12.2559626

KEYWORDS: Tissues, Skin, Cornea, Collagen, Multiphoton microscopy, 3D image processing, Second-harmonic generation, Harmonic generation, Biopsy, Image processing

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Collagen is the major component of connective tissues, where it assembles into fibrils that exhibit various sizes and form various 3D structures depending on the observed tissue. Any disruption of this microstructure is associated with tissue malfunction and defective biomechanical properties. This study thus aims to investigate the relationship between the microstructure and the macroscopic mechanical response of typical connective tissues: skin (disordered) and cornea (highly ordered). To overcome technical issues in providing experimental multiscale data in intact tissues, we have implemented an original setup combining mechanical assays at tissue scale and Second Harmonic Generation (SHG) imaging of collagen reorganization. This multiphoton imaging modality represents an effective structural probe of the micrometer-scale collagen organization in unstained tissues. 3D SHG images were acquired in dermis from ex vivo murine skin biopsies during controlled stretching until rupture, and in ex vivo Human cornea during inflation assays. Specific image processing was implemented to quantify the reorganization of tissue microstructure and correlate it with stress/stretch relationship at macroscopic scale. In murine skin, we showed that the collagen fibers continuously aligned with stretch, generating the observed increase in mechanical stress, which challenges the usual theoretical explanation of the microstructural origin of the skin macroscopic mechanical response. Moreover, dermis from transgenic mice with defective collagen microstructure exhibited altered collagen reorganization upon traction, which could be linked to the microstructural modifications. In Human cornea, our results showed no reorganization of the collagen fibrils at sub-micrometer scale within the stromal lamellae, while the lamellae at micrometer scale reorganized in a more balanced way along 2 main perpendicular directions, in line with the deformation observed at the macroscopic scale. In conclusion, our approach provides an efficient tool to investigate the biomechanics of collagen-rich tissues in normal and pathological context and to guide tissue engineering with appropriate biomechanical responses.

SPIE Journal Paper | 22 June 2016 Open Access

Special Section Guest Editorial:Antonello De Martino (1954–2014): in memoriam

Tatiana Novikova, Bernard Drévillon, Laurent Schwartz, Pierre Validire, André Nazac, Razvigor Ossikovski, Enric Garcia-Caurel, Blandine Laude-Boulesteix, Makrina Anastasiadou, Maria Losurdo, Kurt Hinderl, Bicher Haj Ibrahim, Maria Rosaria Antonelli, Angelo Pierangelo, Stanislas Deby, Stéphane Roussel, Sandeep Manhas, Jérémy Vizet, Dominique Pagnoux, Stéphane Bancelin, Marie-Claire Schanne-Klein, Jean Rehbinder, Huda Haddad, François Moreau, Jean-Charles Vanel, Pere Roca i Cabarrocas, Valery Tuchin, Steven Jacques

JBO, Vol. 21, Issue 07, 071101, (June 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.7.071101

KEYWORDS: Polarimetry, Biomedical optics, Physics, Infrared radiation, Cervical cancer, Head, Interfaces, Thin films, Scientific research, Quantum optics

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Proceedings Article | 5 March 2015 Paper

Second harmonic generation quantitative measurements on collagen fibrils through correlation to electron microscopy

S. Bancelin, C. Aimé, Ivan Gusachenko, Laura Kowalczuk, G. Latour, T. Coradin, M.-C. Schanne-Klein

Proceedings Volume 9329, 93292M (2015) https://doi.org/10.1117/12.2077958

KEYWORDS: Second-harmonic generation, Collagen, Transmission electron microscopy, Tissues, Calibration, Microscopy, Harmonic generation, Electron microscopy, Cornea, Signal processing

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