Studying fiber architectures in tumor tissues is of great importance as the cancer cells interact with fiber structures in the Extracellular Matrix (ECM). Computational Scattered Light Imaging (ComSLI) represents an innovative, non-destructive approach to whole-slide imaging with micrometer resolution, uniquely capable of accurately unraveling the complex, intertwined fiber structures in biological tissues. So far, it has been mainly used to study highly interwoven nerve fiber architectures in brain tissues. In this study, we extend the application of ComSLI to visualize fibers in diverse tumor tissues, including oral squamous cell carcinoma (OSCC) and colorectal cancer.
Computational Scattered Light Imaging (ComSLI) is a novel, non-destructive, whole-slide imaging method with the unique ability to precisely disentangle densely interwoven fiber structures in biological tissues. ComSLI can be performed on microscopy slides regularly prepared within the histopathological routine. Although it is a label-free method and does not require any staining, it also works on stained tissues for various stains. So far, ComSLI has been used to visualize nerve fibers in brain tissues [1-3]. In this study, we visualize muscle and collagen fibers in oral tissues for the first time.
REFERENCES:
[1] Menzel, M., et al. "Using light and X-ray scattering to untangle complex neuronal orientations and validate diffusion MRI." Elife 12 (2023).
[2] Menzel, M., et al. "Scattered Light Imaging: Resolving the substructure of nerve fiber crossings in whole brain sections with micrometer resolution." NeuroImage 233 (2021).
[3] Menzel, M., et al. "Toward a high-resolution reconstruction of 3D nerve fiber architectures and crossings in the brain using light scattering measurements and finite-difference time-domain simulations." Physical Review X 10.2 (2020).
In oral cavity cancer only 15% of operations succeed in removing the whole tumor with the required margin of more than 5 mm of surrounding healthy tissue. This negatively affects prognosis. Clearly the hands and eyes of the surgeon do not suffice.
We use a fiber optic needle probe for high-wavenumber Raman spectroscopic analysis of the freshly resected tissue to determine if the distance between the resection surface and the tumor is sufficient.
A system is under development to inspect the resected tissue in under 15 minutes, while the patient is still in the OR.
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