In this paper, a novel microscopic imaging technology, termed light field Fourier ptychographic microscopy (LFFPM), is presented which enables the reconstruction of wide-field, high-resolution images with the depth information by utilizing a light-emitting-diode (LED) illumination matrix.Conventional light field microscope lacks the ability of high lateral resolution because it sacrifices its lateral resolution to its angular resolution. Compared to conventional light field microscope, LFFPM enhances the lateral resolution with no need for the object to be in focus. Using LFFPM, the result shows that the lateral resolution is more than 2 times better than light field microscopy in simulation and 1.5 times in the experiment. Furthermore, the simulation result also shows that the ability of LFFPM to recovery the information of the phase and amplitude.Thus, this technology has a wide prospect of various biomedical applications such as neuroanatomy and haematology.
A stable optical system is required to acquire a high-quality image. A motionless lensless setup is designated to obtain high-resolution and large field of view images. The sample is sequentially illuminated with multiple random phase patterns, and the recorded images are subtracted from the system calibration images correspondingly. The resultant images are propagated to the sample plane. The summation of all images yields a final image with resolution of ∼4 μm, field of view of ∼15 mm2, and better signal-to-noise ratio. This technique provides a compact, stable, and cost-effective optical system.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.