To enhance the functional image-guided capabilities in preclinical radiotherapy research, we developed fluorescence tomography (FT) and integrated it with a commercial bioluminescence tomography (BLT) system for small animal irradiators. We devised a novel method to achieve submillimeter accuracy in mapping the excitation sources and fluorescence data to the imaged animal surface. We expect to achieve approximately 1 mm accuracy in 3D target localization for in vivo tumor model. The multi-model FT/BLT-guided system will provide advanced image-guided capabilities to enhance preclinical radiotherapy research, particularly in cases where radiation is combined with other systemic treatments, thus expanding its applications beyond local therapy.
Bioluminescence tomography (BLT), as an available image-guided system for pre-clinical radiotherapy research, can localize in vivo tumors within high localization accuracy but it is still challenging to recover accurate structure information due to optical diffusion and ill-posed inverse problem. Recognition of this challenge led us develop novel reconstruction method, optimized spectral-derivative compressive sensing conjugate gradient algorithm. We will perform simulation and in vivo experiments to test BLT’s performance in reconstructing the target location and shape both in primary tumor or metastatic setting. We expect that our BLT-guided system will provide investigators quantitative tumor imaging to perform high precision radiation research.
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