In recent years, transcorneal electrical stimulation(TES)has been regarded as a potential treatment method for degenerative retinal disease. However, the mechanism of TES therapy is still not understood till now. As one of the manifestations of retinal degenerative diseases, the fundus non-vascular perfusion area has been shown to be related to the degeneration of retinal photoreceptor cells and the attenuation of the retinal vasculature and there is a great probability to be cured by TES treatment. The purpose of this study was to analyze the variation of the intrinsic optical signal (IOS) characteristic and the retinal blood vasculature induced by TES in mice and to investigate the therapeutic mechanism of TES for retinal degenerative diseases. In this study, swept-source optical coherence tomography (SS-OCT) system was custom-built to record the IOS and fundus vascular response under TES in pre-, during, and post-stimulation periods, respectively. Results showed that the vessel density (VD) of retinal vessels slightly increased under TES, positive and negative IOS changes significantly increased in all retinal layers, and recovery of the microvascular access in the lesion area was obviously observed. This study might be useful to understanding the treatment mechanism of TES on degenerative retinal diseases and it proved that OCT and OCTA could be used as monitoring techniques for TES therapy.
Multimodal imaging photoacoustic microscopy (PAM) and optical coherence tomography (OCT) can provide morphological and functional information of living organisms which reveals the physiological and pathological state of biological tissues. In this study, a dual-modal PAM and OCT system was custom-built to simultaneously image the optical absorption and scattering characteristic of rabbit eyes. Glycerol solution was used as optical clearing agent to enhance PAM and OCT imaging of New Zealand White rabbit eyes in vivo. Results showed that both OCT and PAM signals were increased by retro-bulbar injection of glycerol to New Zealand White rabbit eyes. The PAM signal amplitude increased from 0.1476 (a.u) to 0.1701 (a.u) after glycerol solution injection, which increased by 115%. And intensity of OCT signal was increased up to 150%. The optical clearing agent enhanced multimodal imaging technique provides a new method to increase the depth of optical measured depth and to improve the imaging contrast, which can be used to evaluate pathophysiological early symptoms in vivo.
Myopia is a significant cause of visual impairment which may lead to many complications. Though the mechanism of myopia is not clear, the changes of the biological parameters and the optical performance of eyes were suggested as a key mechanism. In order to investigate the mechanism of myopia, in this paper, the time serial evaluation of biological parameters was performed and the optical performance of myopic model mouse eyes were evaluated by using optical coherence tomography (OCT) and ZEMAX software. The biological parameters were compared, and the optical performance of mouse eyes were evaluated in the myopic model mouse eyes, normal control mouse eyes and atropine-treated mouse eyes. The correlation analysis of the statistics indicated that the development of myopia was significantly related to the corneal curvature radius, center corneal thickness, vitreous depth and axial length (P<0.05). The analysis of biological parameters proved that the peripheral RMS wavefront aberration of eyes played more crucial roles in the development of myopia than the center parameters, and the degree of myopia would be proofed by the hyperopic wavefront aberration. The myopic model mouse eyes had larger corneal curvature radius, thicker center corneal thickness, deeper vitreous depth and longer axial length than normal mouse eyes. The experimental results of atropine-treated eyes suggested that atropine could relieve myopia and eye enlargement via a nonaccommodative mechanism. This study provides a new method for evaluations of the optical performance of eyes and the study of myopia.
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