Large-scale neural interfaces for optogenetic actuators and sensors in non-human primates

Devon J. Griggs; Tiphaine Belloir; Azadeh Yazdan-Shahmorad

doi:10.1117/12.2579431

5 March 2021 Large-scale neural interfaces for optogenetic actuators and sensors in non-human primates

Devon J. Griggs, Tiphaine Belloir, Azadeh Yazdan-Shahmorad

Proceedings Volume 11663, Integrated Sensors for Biological and Neural Sensing; 1166305 (2021) https://doi.org/10.1117/12.2579431
Event: SPIE BiOS, 2021, Online Only

Abstract

Optogenetics is a powerful tool for relating brain function to behavior, as it enables cell-type specific manipulation and recording of neurons with high spatial and temporal precision. Although optogenetics has been used successfully in nonhuman primates, reliable techniques had not been developed for large-scale, bi-directional study of neural circuits in these animals. Here we present practical and stable interfaces for stimulation and recording of large-scale cortical circuits. To obtain optogenetic expression across a broad region, spanning large cortical areas (5 cm2 ), we used convection-enhanced delivery of the viral vector, with online guidance from magnetic resonance imaging. To record neural activity across this region, we used micro-electrocorticographic (μECoG) arrays designed to minimally attenuate optical stimuli. Lastly, we have incorporated the capability of producing focal and modular photochemical ischemic lesions in these interfaces enabling us to stimulate the cortex around the site of injury and monitor functional recovery via change in blood flow, neurophysiology and behavior. These interfaces offer powerful tools for studying circuit dynamics and connectivity across cortical areas, for long-term studies of neuromodulation, and for linking these to behavior. Currently we are using these technologies towards developing therapeutic interventions for neurological disorders such as stroke.

Conference Presentation

Citation Download Citation

Devon J. Griggs, Tiphaine Belloir, and Azadeh Yazdan-Shahmorad "Large-scale neural interfaces for optogenetic actuators and sensors in non-human primates", Proc. SPIE 11663, Integrated Sensors for Biological and Neural Sensing, 1166305 (5 March 2021); https://doi.org/10.1117/12.2579431

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