Nonlinear focal modulation microscopy based on interleaved reconstruction

Yuchen Chen; Chengfeng Zhang; Cuifang Kuang

doi:10.1117/12.2537324

19 November 2019 Nonlinear focal modulation microscopy based on interleaved reconstruction

Yuchen Chen, Chengfeng Zhang, Cuifang Kuang

Proceedings Volume 11186, Advanced Optical Imaging Technologies II; 1118611 (2019) https://doi.org/10.1117/12.2537324
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Nonlinear focal modulation microscopy (NFOMM) is a really simple and effective super-resolution imaging method based on phase modulation with high-intensity illumination, which can extend the effective spatial-frequency bandwidth of the imaging system and achieve a transverse resolution of ~ 60 nm (~λ/10). However, multiple images under different illumination pattern is needed, which result in limited imaging speed. A novel interleaved reconstruction method is proposed to increase the frame rate without any change in the raw data of NFOMM. Since allowing easy integration with confocal microscopes, we anticipate this method will be a useful observation tool in the biological community and other research field.

Citation Download Citation

Yuchen Chen, Chengfeng Zhang, and Cuifang Kuang "Nonlinear focal modulation microscopy based on interleaved reconstruction", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 1118611 (19 November 2019); https://doi.org/10.1117/12.2537324

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopy

Modulation

Reconstruction algorithms

Point spread functions

Confocal microscopy

Optical transfer functions

Deconvolution

Show All Keywords

Keywords/Phrases

Search In:

Publication Years