An LED-based super resolution GPU implemented structured illumination microscope

Musa Aydin; Yiğit Uysallı; Ekin Özgönül; Berna Morova; Alper Kiraz

doi:10.1117/12.2544602

13 February 2020 An LED-based super resolution GPU implemented structured illumination microscope

Musa Aydin, Yiğit Uysallı, Ekin Özgönül, Berna Morova, Alper Kiraz

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 1124610 (2020) https://doi.org/10.1117/12.2544602
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel- lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of ∼150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

Citation Download Citation

Musa Aydin, Yiğit Uysallı, Ekin Özgönül, Berna Morova, and Alper Kiraz "An LED-based super resolution GPU implemented structured illumination microscope", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124610 (13 February 2020); https://doi.org/10.1117/12.2544602

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