Quantitative polymerase chain reaction using photothermal conversion of Au nanoshell for point-of-care diagnostics

Jae Sung Ahn; Woongyu Park; Sun-Hee Ahn

doi:10.1117/12.3002102

27 March 2024 Quantitative polymerase chain reaction using photothermal conversion of Au nanoshell for point-of-care diagnostics

Jae Sung Ahn, Woongyu Park, Sun-Hee Ahn

Author Affiliations +

Proceedings Volume 12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics; 1285007 (2024) https://doi.org/10.1117/12.3002102
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The global rise of infectious diseases underscores the urgent need for rapid, accurate diagnostics. Our study introduces a novel polymerase chain reaction technique utilizing gold nanoshells for faster and more efficient DNA amplification. These nanoshells' unique properties enable rapid heating in the near-infrared spectrum, accelerating the PCR process by quickly reaching optimal temperatures. This approach streamlines DNA amplification and ensures detection of small DNA quantities. Integrating photothermal PCR with advanced real-time fluorometry and non-invasive temperature monitoring, we can amplify DNA in just 25 minutes and detect as little as 50 picograms. The use of gold nanoshells' heating capabilities leads to quicker, more sensitive DNA detection. This innovation is a significant advancement in PCR technology, especially for point-of-care diagnostics, promising quick identification of DNA markers vital for prompt infectious disease diagnosis. It's particularly valuable in urgent care or resource-limited settings, where rapid, reliable results are essential.

Conference Presentation

Citation Download Citation

Jae Sung Ahn, Woongyu Park, and Sun-Hee Ahn "Quantitative polymerase chain reaction using photothermal conversion of Au nanoshell for point-of-care diagnostics", Proc. SPIE 12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics, 1285007 (27 March 2024); https://doi.org/10.1117/12.3002102

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