Presentation + Paper
27 March 2024 Quantitative polymerase chain reaction using photothermal conversion of Au nanoshell for point-of-care diagnostics
Jae Sung Ahn, Woongyu Park, Sun-Hee Ahn
Author Affiliations +
Abstract
The global rise of infectious diseases underscores the urgent need for rapid, accurate diagnostics. Our study introduces a novel polymerase chain reaction technique utilizing gold nanoshells for faster and more efficient DNA amplification. These nanoshells' unique properties enable rapid heating in the near-infrared spectrum, accelerating the PCR process by quickly reaching optimal temperatures. This approach streamlines DNA amplification and ensures detection of small DNA quantities. Integrating photothermal PCR with advanced real-time fluorometry and non-invasive temperature monitoring, we can amplify DNA in just 25 minutes and detect as little as 50 picograms. The use of gold nanoshells' heating capabilities leads to quicker, more sensitive DNA detection. This innovation is a significant advancement in PCR technology, especially for point-of-care diagnostics, promising quick identification of DNA markers vital for prompt infectious disease diagnosis. It's particularly valuable in urgent care or resource-limited settings, where rapid, reliable results are essential.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jae Sung Ahn, Woongyu Park, and Sun-Hee Ahn "Quantitative polymerase chain reaction using photothermal conversion of Au nanoshell for point-of-care diagnostics", Proc. SPIE 12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics, 1285007 (27 March 2024); https://doi.org/10.1117/12.3002102
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KEYWORDS
Polymerase chain reaction

Gold

Diagnostics

Fluorescence

Point-of-care devices

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