Cytogenetic analysis of interphase cells using spectral imaging [SIm] technology

Heinz-Ulli G. Weier; Jan Smida; Horst Zitzelsberger; Robert A. Lersch; Jamie Hung; H. Ben Hsieh; K. Salassidis; George McNamara; Roger A. Pedersen; Jingly Fung

doi:10.1117/12.379585

14 March 2000 Cytogenetic analysis of interphase cells using spectral imaging [SIm] technology

Heinz-Ulli G. Weier, Jan Smida, Horst Zitzelsberger, Robert A. Lersch, Jamie Hung, H. Ben Hsieh, K. Salassidis, George McNamara, Roger A. Pedersen, Jingly Fung

Author Affiliations +

Proceedings Volume 3920, Spectral Imaging: Instrumentation, Applications, and Analysis; (2000) https://doi.org/10.1117/12.379585
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Spectral Imaging (SIm) has dramatically improved our ability to localize and quantitatively analyze multiple nucleic acid targets such as chromosomes, genes and gene transcripts. Studies on metaphase cells such as `Spectral Karyotyping' are less complicated than interphase cell studies because the objects (chromosomes) are spatially separated and ratio- labeled probes can be used to uniquely stain each chromosome type. Our research, however, targets the extensive cytogenetic and phenotypic analysis of interphase cells. The complex organization of interphase chromatin and co- localization of gene transcripts (RNAs) in nuclear or cytoplasmic domains requires unique fluorochrome-labeling for each nucleic acid target. An increasing number of commercially available dyes for probe labeling and software to deconvolute partially overlapping emission spectra has helped to overcome most of these obstacles. This presentation summarizes our experience in analyzing numerical and structural alterations in various human cell types (leukocytes, amniocytes, blastomeres or solid tissue) as well as our approach to multi-gene expression profiling using SIm. Examples illustrate a wide spectrum of groundbreaking techniques for interphase cell analysis. We demonstrate how ten or more chromosomes can be scored in interphase nuclei or the relative level of expression of different transforming RNAs in tumor cells can be measured by SIm.

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Heinz-Ulli G. Weier, Jan Smida, Horst Zitzelsberger, Robert A. Lersch, Jamie Hung, H. Ben Hsieh, K. Salassidis, George McNamara, Roger A. Pedersen, and Jingly Fung "Cytogenetic analysis of interphase cells using spectral imaging [SIm] technology", Proc. SPIE 3920, Spectral Imaging: Instrumentation, Applications, and Analysis, (14 March 2000); https://doi.org/10.1117/12.379585

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