Lysosomal photodamage induces cell death via mitochondrial apoptotic pathway

Lei Liu; Xian-wang Wang; Hui Li

doi:10.1117/12.838110

2 December 2009 Lysosomal photodamage induces cell death via mitochondrial apoptotic pathway

Lei Liu, Xian-wang Wang, Hui Li

Proceedings Volume 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging; 75070I (2009) https://doi.org/10.1117/12.838110
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

Lysosomal photosensitizers have been used in photodynamic therapy (PDT). Combination of such photosensitizers and light causes lysosomal photodamage, inducing cell death. The lysosomal disruption can lead to apoptosis but its signaling pathways remain to be elucidated. In this study, we selected N-aspartyl chlorin e6 (NPe6), an effective photosensitizer which preferentially accumulates in lysosomes, to study the mechanism of apoptosis caused by lysosomal photodamage. Apoptosis in living human lung adenocarcinoma cells treated by NPe6-PDT was studied using real-time single-cell analysis. In this study, the fluorescence probes Cyto c-GFP and DsRed-Mit were used to detect the spatial and temporal changes of cytochrome c in real-time in sub-cell level; the Rhodamine 123 dyes were used to monitor the changes of mitochondrial membrane potential. The results showed that, after PDT treatment,the mitochondrial membrane potential decreased, and cytochrome c released from mitochondria; The caspase-3 was activated obviously. These results suggested that lysosomal photodamage activates mitochondrial apoptotic pathway to induce cell death.

Citation Download Citation

Lei Liu, Xian-wang Wang, and Hui Li "Lysosomal photodamage induces cell death via mitochondrial apoptotic pathway", Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070I (2 December 2009); https://doi.org/10.1117/12.838110

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