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9 March 2023 Rapid virtual histology of fresh tissue via photoacoustic remote sensing microscopy and a voice-coil scanning stage
Brendyn D. Cikaluk, Brendon S. Restall, Matthew T. Martell, Nathaniel J. M. Haven, Roger J. Zemp
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Proceedings Volume PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023; PC123791V (2023) https://doi.org/10.1117/12.2649205
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We demonstrate a rapid photoacoustic remote sensing (PARS) microscopy system with voice-coil stage scanning, capable of acquiring 1cm2 virtual histology images at 800nm x 800nm lateral sampling density in 3.75 minutes, high-resolution (300nm x 300nm sampling density) images at 1mm2 in 20 seconds and 300μm x 300μm in 3 seconds. The combined resolution and scanning speed that can be achieved by this system may pave way for clinical translation to rapidly assess resected tumor margins in the surgical suite.
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Brendyn D. Cikaluk, Brendon S. Restall, Matthew T. Martell, Nathaniel J. M. Haven, and Roger J. Zemp "Rapid virtual histology of fresh tissue via photoacoustic remote sensing microscopy and a voice-coil scanning stage", Proc. SPIE PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023, PC123791V (9 March 2023); https://doi.org/10.1117/12.2649205
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KEYWORDS
Microscopy
Photoacoustic spectroscopy
Remote sensing
Tissue optics
Imaging systems
Ultraviolet radiation
Scattering
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