KEYWORDS: Microscopy, Luminescence, Motion models, Imaging systems, Image resolution, Real time imaging, Performance modeling, In vivo imaging, Image filtering, Human subjects
Mucin secretive conjunctival goblet cells (CGCs) in the eye are important for tear film stability and ocular surface health. Because CGC dysfunction is associated with various ocular surface diseases, non-invasive CGC examination will be of great help in the diagnosis and treatment. In this study, we developed a high-speed moxifloxacin-based extended depth-of-field microscopy for real-time CGC examination. The performance was demonstrated by high-speed CGC imaging of both mouse and rabbit models, in vivo. The imaging was insensitive to breathing motion, and the image resolution was sufficient to resolve individual CGCs in rabbit models.
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