A new method for non-invasive determination of optical parameters (absorption and reduced scattering coefficients) of biological tissues from diffuse reflectance measurements with a multifiber probe is presented. For extraction of the parameters, we use a priori estimations not only of signals of detectors, but also their parameter derivatives that increases accuracy of the extraction. This estimation is performed using a new non-analog Monte Carlo algorithm which allows us to calculate the signals and derivatives simultaneously for a given large set of optical parameters. Experimental testing has shown the measured coefficients provide a good prediction of both light reflection and penetration. Application of the method to some biological tissues are presented.
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