Ultrashort laser pulses are very promising tools for performing accurate dissection in the eye, especially in the corneal stroma. The development of eye femtosurgery requires basic knowledge about laser-tissue interaction. One of the most significant parameters is the ablation threshold, the minimal laser energy per unit surface required for ablation. We present here measurements of the femtosecond laser ablation threshold as a function of the pulse duration for two cornea layers (epithelium and stroma) using optical damage diagnosis. Experiments have been realized with the INRS Ti:Sapphire laser (60 fs-5000 fs, 800 nm, 10 Hz). Our experimental results are fitted with a model for laser-matter interaction in order to determine some intrinsic physical parameters
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