Ms. Fan Yang Profile

Fan Yang

at Beijing Institute of Technology

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 27 November 2023 Poster + Paper

Functional platelet aggregation analysis using digital optofluidic scattering quantitation

Shan Qin, Rongxin Fu, Fan Yang, Weibo Gao, Jialu Hou, Hang Li, Shuailong Zhang

Proceedings Volume 12770, 127701K (2023) https://doi.org/10.1117/12.2685606

KEYWORDS: Image processing, Diseases and disorders, Optofluidics, Plasma

Read Abstract +

Platelet aggregation rate is a crucial indicator for platelet function testing in clinical practice. Currently, the most common method for detecting platelet aggregation rate is by using a platelet aggregation analyzer to detect platelet-rich plasma or washed platelets. This method works based on the principle of light transmittance ratio, which measures the difference of the light transmittance before and after the reaction between the sample and the inducer to represent the aggregation rate of the sample. Although this method only requires a few minutes to complete after the sample is collected and processed, it requires a large sample volume. Moreover, it cannot accurately detect samples with low aggregation rate and chyle blood due to the slight difference in light transmittance before and after the reaction, which poses significant challenges for doctors in the clinical diagnosis and treatment determination for platelet aggregation dysfunction. To overcome this limitation, we propose a novel method for assessing platelet aggregation rate by leveraging the capabilities of digital microfluidics for automated precision manipulation of minute liquid droplets.

Proceedings Article | 27 November 2023 Poster + Paper

Label-free recombinase polymerase amplification with hyperspectral digital optofluidics

Tianqi Zhou, Xiangyu Jin, Fan Yang, Shan Qin, Fenggang Li, Shuailong Zhang, Hang Li, Rongxin Fu

Proceedings Volume 12770, 127701G (2023) https://doi.org/10.1117/12.2684913

KEYWORDS: Nucleic acids, Microfluidics, Molecules

Read Abstract +

Nucleic acid detection techniques have played a crucial role in identifying specific genetic indicators or species, with Polymerase Chain Reaction (PCR) being the established gold standard in this field. However, PCR's dependence on specialized equipment and skilled personnel has limited its utility in resource-limited or field settings, and its multitemperature stage protocol hinders rapid nucleic acid detection. The emergence of isothermal amplification methods, particularly recombinase polymerase amplification (RPA), has addressed some of these limitations, offering high sensitivity and efficiency. Nevertheless, the challenge of RPA amplicon detection, typically reliant on labeling methods, has persisted, potentially introducing false positives and increased costs. This study introduces an innovative approach to nucleic acid detection, harnessing hyperspectral quantitative interference for label-free, isothermal nucleic acid detection within a remarkably short 25-minute timeframe. By employing a solid-phase RPA amplification process that transforms the product into a DNA molecule layer and leveraging Fourier domain optical slice separation and spectral phase shift analysis, this method enables the semi-quantitative determination of amplification results. The integration of digital microfluidic technology further enhances the method's performance, enabling parallel, integrated, and clinical multi-indicator pathogen detection. Overall, this research presents a practical and rapid solution for label-free nucleic acid detection, addressing the current limitations associated with nucleic acid amplification techniques. This advancement holds promise for a wide range of applications, from point-of-care diagnostics to field-based pathogen detection, ultimately contributing to more accessible and efficient nucleic acid testing methodologies.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years