Here we explore the use of Photoacoustic Remote Sensing (PARS™) microscopy, a recently developed non-contact photoacoustic imaging modality, for visualizing subcellular structures label-free in tissues. Operating in an all-optical reflection-mode architecture PARS captures optical absorption contrast within bulk tissue samples. Presented here, by visualizing endogenous optical absorption of DNA and cytochromes, cellular morphology is captured with contrast analogous to the industry standard hematoxylin and eosin (H&E) staining. Subcellular features are recovered from human and murine, brain and gastrointestinal tissues. This work represents a significant step towards the development of a real-time microscopy system for label-free histopathological assessment of tissues in-situ.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.