Mr. Haoyu Wang Profile

Haoyu Wang

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Proceedings Article | 2 March 2022 Presentation + Paper

Feasibility demonstration of supercontinuum fiber laser-based coherent anti-Stokes Raman scattering microscopy

Jiaojiao Zhang, Nan Wang, Haoyu Wang, Wangting Zhou, Xinyi Xu, Qi Zeng, Xueli Chen

Proceedings Volume 11973, 1197307 (2022) https://doi.org/10.1117/12.2608358

KEYWORDS: Fiber lasers, Microscopes, Microscopy, Raman spectroscopy, Imaging systems

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Coherent anti-Stokes Raman scattering (CARS) microscopy enables the analysis of the chemical composition and distribution within living cells, biomolecules, or living organisms in a label-free manner. Compared with the traditional spontaneous Raman imaging technology, its advantages of high imaging sensitivity and resolution, fast imaging speed and strong signal intensity make it more popular in multiple disciplines. The available CARS microscopes are most adopted advanced crystal solid-state lasers, which are expensive, bulky, and sensitive to the environmental changes. Supercontinuum fiber lasers with a wide spectral tuning range are increasingly used in biomedical applications due to their low cost, small size, and low environmental impact. Here, we homebuilt a CARS microscope based on a supercontinuum fiber laser, a specially tailored laser with a dual-channel time-synchronous outputs. The influence factors were investigated including the objective numerical aperture, laser power, and sample concentration, etc. The feasibility of CARS microscope was then verified by imaging the polystyrene microspheres (PS) and polymethyl methacrylate microspheres (PMMA). Finally, we imaged the lipid droplet distribution of EC109 cell, which revealed the application potential of the supercontinuum fiber laser-based CARS microscope in biomedical applications.

Proceedings Article | 21 February 2020 Presentation + Paper

Feasibility study of the portable Raman spectroscopy based on Bessel beam

Feng Ren, Haoyu Wang, Nan Wang, Yichao Liu, Yonghua Zhan, Xueli Chen

Proceedings Volume 11252, 112521D (2020) https://doi.org/10.1117/12.2543974

KEYWORDS: Raman spectroscopy, Spectroscopy, Bessel beams, Raman scattering, Scattering, Light scattering, Signal to noise ratio, Chemical analysis

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Traditional drug detection technique is highly accurate but time consuming and labor intensive. Raman spectroscopy (RS) is a fast and non-destructive detection technique that provides detailed information on chemical composition, phase and morphology, crystallinity and molecular interaction of the sample. The current Raman spectrometer is mainly based on the use of Gaussian light, providing with good signal to noise ratio for a thin or transparent sample. However, owing to the scattering effect, the Gaussian beam will become diffuse in the scattering medium. This makes it not conducive to in vivo or deep imaging. Utilizing the long focusing characteristics and self-reconstructing properties of Bessel beam, we here presented a new scheme for RS, which used a Bessel beam as the excitation light. The Bessel beam-based RS was first verified with the standard samples, and then comparatively tested on several drugs. Taking the acetaminophen as the test sample, we compared the Bessel beam-based RS with the traditional Gaussian beam based one with or without a scattering medium. With the addition of a scattering medium, the signal-to-noise ratio of Raman spectra based on Bessel beam decreases less than that based on the Gaussian light, which demonstrated the great potential of the use of Bessel beam in in vivo or deep RS. This study provides great value for in vivo applications of Raman spectroscopy.

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