In this work, we applied multi-photon microscopy to image and characterize the extent of tissue
glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic
generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of
providing qualitative and quantitative information of the extent of tissue glycation in ribose treated
samples including bovine sclera and vessel and that this approach may be useful for clinical
evaluation of advanced glycation endproducts (AGEs) formation.
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