With the development of multilayer models for the analysis of quantitative spectroscopic techniques, there is a need
to generate controlled and stable phantoms capable of validating these new models specific to the particular
instrument performance and/or probe geometry. Direct applications for these multilayer phantoms include
characterization or validation of depth penetration for specific probe geometries or describing layer specific
sensitivity of optical instrumentation.
We will present a method of producing interchangeable silicone phantoms that vary in thickness from 90 microns up
to several millimeters which can be combined to produce multilayered structures to mimic optical properties of
physiologic tissues such as skin. The optical properties of these phantoms are verified through inverse addingdoubling
methods and the homogeneous distribution of optical properties will be discussed.
Currently the only method for positively identifying malignant melanoma involves invasive and often undesirable biopsy
procedures. Available ex-vivo data indicates increased vascularization in the lower regions of excised melanoma, as
compared to dysplastic nevi. The ability to interrogate this region of tissue in-vivo could lead to useful diagnostic
information. Using a newly developed fiber based superficial probe in conjunction with a steady-state frequency-domain
photon migration (SSFDPM) system, we can probe the upper 1-2 mm of tissue, extracting functional information in the
near infrared (650-1000 nm) range. To test the resolution and detection range of the superficial probe in this context,
deformable silicone phantoms have been fabricated that simulate normal skin with melanocytic lesions. These phantoms
consist of a two-layered matrix with the optical properties of normal light skin, containing several cylindrical inclusions
that simulate highly absorbing pigmented lesions such as melanoma. These inclusions are varied in depth, diameter, and
optical properties in order to fully test the probe's detection capabilities. It was found that, depending on absorption, we
can typically probe to a depth of 1.0-1.5 mm in an inclusion, likely reaching the site of angiogenesis in an early-stage
melanoma. Additionally, we can successfully interrogate normal tissue below lesions 1.5mm deep when absorption is
about 0.4/mm or less. This data indicates that the superficial probe shows great promise for non-invasive diagnosis of
pigmented lesions.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.