A Complementary Metal Oxide Semiconductor (CMOS) camera (1024×1024 pixels) is used to record spontaneous
oscillations of hair cell stereocillia in an in-vitro preparation of the bullfrog sacculus with the otolithic membrane
removed. The CMOS camera is attached to an Olympus BX51WI Microscope inside of a sound-isolation chamber, with
white light transmission illumination using an X-Cite 120 metal halogenide lamp. The combination of the parallel
readout of the CMOS chip and the high intensity of illumination allows full frame images of the oscillations to be taken
at 1000 frames per second. A weighted, time averaged differential algorithm is used to aid in the visualization of the hair
cell movement. To detect the displacement from its center of the stereocillia tip with nanometer position resolution and
millisecond time resolution, an average background intensity value was subtracted from each image to remove lamp
intensity fluctuations and then a center of intensity algorithm was applied. This combination of our imaging system and
data analysis allows for the oscillations of more than one hair cell to be recorded during the same time period, and their
frequency components extracted.
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