This paper concerns on the Optical Transfer Function (OTF) of confocal fluorescent microscope in laser scanning microarray system. The cut-off of confocal microscopy, the lateral and the lognitudinal resolution, and the relationship of pinhole and resolution were discussed in a novel method. At the same time, all of these parameters of confocal microscope in microarray system are compared with other conventional and coherent microscope. The results obtained in this paper are significant for selecting the appropriate parameters and evaluating the properties of a confocal scanning fluorescent microscope for microarray reader system in order to improve the performance.
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