Dr. Peng Guo Profile

Dr. Peng Guo

Instructor in Surgery at Boston Children's Hospital

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Proceedings Article | 23 February 2018 Paper

Quantitative phase imaging characterization of tumor-associated blood vessel formation on a chip

Peng Guo, Jing Huang, Marsha Moses

Proceedings Volume 10503, 105031O (2018) https://doi.org/10.1117/12.2289006

KEYWORDS: Microfluidics, Blood vessels, In vitro testing, Curium, Phase imaging, In vivo imaging, Microscopy, Visualization, Phase contrast

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Angiogenesis, the formation of new blood vessels from existing ones, is a biological process that has an essential role in solid tumor growth, development, and progression. Recent advances in Lab-on-a-Chip technology has created an opportunity for scientists to observe endothelial cell (EC) behaviors during the dynamic process of angiogenesis using a simple and economical in vitro platform that recapitulates in vivo blood vessel formation. Here, we use quantitative phase imaging (QPI) microscopy to continuously and non-invasively characterize the dynamic process of tumor cell-induced angiogenic sprout formation on a microfluidic chip. The live tumor cell-induced angiogenic sprouts are generated by multicellular endothelial sprouting into 3 dimensional (3D) Matrigel using human umbilical vein endothelial cells (HUVECs). By using QPI, we quantitatively measure a panel of cellular morphological and behavioral parameters of each individual EC participating in this sprouting. In this proof-of-principle study, we demonstrate that QPI is a powerful tool that can provide real-time quantitative analysis of biological processes in in vitro 3D biomimetic devices, which, in turn, can improve our understanding of the biology underlying functional tissue engineering.

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