Colorectal cancer (CRC) ranks third globally in terms of prevalence, accounting for 10% of cancer cases and deaths each year. Irreversible electroporation (IRE) offers promise for treating CRC by selectively destroying tumor cells while minimizing harm to healthy tissue. However, the lack of effective guidance and evaluation methods hampers the achievement of complete tumor ablation. To address this, integrated OCT, US, and NIRF endoscopy enable real-time imaging of colorectal tumors, facilitating precise IRE treatment and evaluation of outcomes. In this study, we used an integrated imaging system to precisely guide and evaluate the implementation of IRE therapy in CRC.
In-stent restenosis (ISR) and its associated inflammation remains a significant concern for long-term patient outcomes following stent implantation in percutaneous coronary intervention (PCI). The problem is intricately associated with endothelial injury, excessive endothelialization, hyperproliferation of smooth muscle cells, and the infiltration of inflammatory molecules. However, commonly employed imaging techniques encounter challenges in simultaneously acquiring both vascular structural information and functional data related to inflammation. Here, we presented a novel Tri modality intravascular imaging system capable of simultaneous optical coherence tomography (OCT), near-infrared fluorescence (NIRF), ultrasound (US) imaging, and fabricated the OCT-NIRF-US catheter which outer diameter is 0.75 mm, aiming to provide a more comprehensive diagnostic tool for ISR and its associated inflammation. Experiments were conducted on atherosclerotic rabbits implanted with a scaffold, divided into two groups (n=3 each group) for assessment on the first and twenty-eighth day, respectively. Primary results demonstrated that the integrated OCT-NIRF-US intravascular system enables complementary structural imaging and functional imaging of inflammation. The system presents the potential to offer a more accurate assessment, providing with valuable insights into the ISR processing and assisting in the development of more precise clinical strategies.
Endoscopic optical coherence tomography (OCT) has been demonstrated for volumetric imaging of subsurface features with high resolution. However, it is difficult to enable endoscopic OCT angiography (OCTA) due to the low inter-frame stability of endoscopic OCT. Recently, stable distal rotational scanning of micromotor catheter enabled imaging of structural features in the en face plane as well as endoscope OCTA. However, most endoscopic OCT in the lab and almost all commercial ones use proximal scanning catheters for diagnosing endoscopic tissues which should be designed much smaller than micromotor catheters. Here, we presented a proximal scanning endoscopic OCT technology that enabled OCTA. A spatiotemporal singular value decomposition (SVD) process was used to remove the eigen components that represented static tissue signals to generate that of the final moving particles. Primary results revealed that the endoscopic imaging system enabled OCTA in the two-and three-dimensional in vitro flow phantom. As the catheter’s outer diameter is less than 1 mm, the system is of potential for providing a more accurate assessment for pancreatic and bile duct cancers and even cardiovascular disease in clinical applications.
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