An infrared fiber-optic neurotoxin biosensor was constructed by applying a biologically active cladding to the core of an infrared transmitting chalcogenide fiber. Binding of the surface bound receptor protein was monitored by performing infrared difference spectroscopy on the fiber-optic probe before and after its exposure to various concentrations of neurotoxin in solution. Signals measuring conformational change(s) as a result of these interactions are observed to saturate in agreement with established biochemical kinetics for the receptor. Fiber-optic components are shown to be much more sensitive than bulk optical components in performing these measurements.
Over the last few years a number of uses of chalcogenide fibers for analyses in the mid infrared have been described. This paper will describe a new approach using a tapered mid infrared fiber as part of a commercial accessory.
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