Fluorescence diffuse optical tomography (FDOT) is an emerging optical imaging tool for in-vivo observation of organisms and small-animal. This modality retrieves the distribution of the concentration of the fluorescent biomarkers noninvasively and quantitatively, which is related to the interior cellular and molecular events. After the FDOT system is established, its performance parameters should be comprehensively and systematically evaluated. This paper conducts an experimental study on the parameters such as spatial resolution and sensitivity of our FDOT system. The fluorescent reagent used in the experiment was indocyanine green, and the phantom material was polyformaldehyde.
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