Mr. Xinyu Wang Profile

Xinyu Wang

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Proceedings Article | 4 March 2019 Paper

Simulation of the stimulated Raman scattering signal generation in scattering media excited by Bessel beams

Xinyu Wang, Yonghua Zhan, Jimin Liang, Xueli Chen

Proceedings Volume 10890, 108900R (2019) https://doi.org/10.1117/12.2508494

KEYWORDS: Bessel beams, Glasses, Beam propagation method, Signal generators, Scattering media, Microscopy, Optical simulations, Gaussian beams, Raman scattering, 3D image processing

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Stimulated Raman scattering (SRS) microscopy has been increasingly used in biology and medicine in recent years due to its ability to image chemical bonds without labelling. Traditional SRS imaging uses Gaussian beams as the excitation sources, which can achieve high spatial and axial resolutions because of the tight focus of the Gaussian beam. However, the tight focus poses serious problems for observing the scattering media. The Gaussian beam would defocus after propagating through a small distance in scattering media. The SRS microscopy cannot work well in this case. Having the self-healing property, Bessel beams may bring solution to this problem. In our previous work, we applied the Bessel beams to the SRS and implemented three-dimensional SRS imaging with projection concept. Here, we simulated the propagation of Bessel beams and the generation of SRS signals with the beam propagation method (BPM). By adding glass beads on the beam propagation path to simulate scatters, the propagation of the Bessel beams and the generation of the SRS signals would change. We designed a series of simulations to investigate the influence of the size and position of the added glass beads to the generation of SRS signals. Simulation results demonstrated that the SRS signals can generate or be recovered at the certain depth in scattering media.

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