Principle, application and prospect of CRISPR-Cas9 regulatory system

Zening Xiao

doi:10.1117/12.2692261

7 September 2023 Principle, application and prospect of CRISPR-Cas9 regulatory system

Zening Xiao

Author Affiliations +

Proceedings Volume 12789, International Conference on Modern Medicine and Global Health (ICMMGH 2023); 127890S (2023) https://doi.org/10.1117/12.2692261
Event: International Conference on Modern Medicine and Global Health (ICMMGH 2023), 2023, Oxford, United Kingdom

Abstract

This article mainly introduces the CRISPR-Cas9 technology, as people all know the cluster spaced short palindromic repetition associated (Cas) protein system, which is an immune mechanism widely present in bacteria and archaea. Because Crisper has the characteristics of efficient and accurate cutting and cracking of foreign DNA at specific sites, it has attracted the attention of many researchers in recent years. This paper mainly introduces the current application and development of CRISPR-Cas9 and its biggest problem — off-target problem, from the aspects of in vivo experiment and gene level, such as the construction of cell models with diseases, gene therapy for thalassemia, cell transfection and tumor. CRISPR-Cas9 can split target DNA even if the sgRNA does not match the target DNA exactly, which limits the further application of genome editing technology, especially with the methods of genetic diseases at non-targeted DNA sites. Therefore, reducing the off-target effect of CRISPR-Cas9 is of great significance for the developments and applications of this technology.

Citation Download Citation

Zening Xiao "Principle, application and prospect of CRISPR-Cas9 regulatory system", Proc. SPIE 12789, International Conference on Modern Medicine and Global Health (ICMMGH 2023), 127890S (7 September 2023); https://doi.org/10.1117/12.2692261

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