A 3D printed microfluidic device for particle sorting was demonstrated using syringe-based fluid flow. Flow speeds of 192 μm/s were shown. This demonstrated an inexpensive, quick and facile fabrication approach to microfluidic particle sorting.
A 3D printed (3DP) microfluidic polymerase chain reaction (PCR) device was demonstrated by detecting synthetic SARS-CoV-2 at 106 copies/μL. The microfluidic device was fabricated using stereolithography 3DP and had a reaction volume of ~22 nL. The microdevice showed PCR amplification with 85 base synthetic ssDNA targets and primers designed for a SARS-CoV-2-specific region. The device was 2.5 times faster compared to a qPCR instrument with >60,000 times smaller reagent volume. The 3DP microdevice is a promising technology to significantly reduce the manufacturing costs of microfluidic devices that could be used towards point-of-care applications.
A 3D printed microfluidic device for Caenorhabditis elegans analysis. The biocompatibility of the 3D printed poly(ethylene glycol) diacrylate hydrogel resin was conducted and had no observable effect on worm lifespans. Worm organ morphology was directly observable through the microfluidic device and low autofluorescence was demonstrated using genetically-modified pharynx fluorescent worms.
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