We previously introduced a novel method called temperature modulated fluorescence tomography (TMFT), which utilizes temperature sensitive ICG loaded pluronic nanocapsules termed ThermoDots and high intensity focused ultrasound (HIFU). TMFT leverages the superior sensitivity of the fluorescence imaging and the high spatial-resolution of focused ultrasound. Previously, we have presented a prototype system using a CCD camera. However, the scan time was too long for in vivo imaging since HIFU was scanned in a step-and-shoot mode. Here, we present the new continuous line scanning scheme, which drastically reduces the imaging time and paves the wave for in vivo preclinical imaging.
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