The function of orthopedic tissues such as cartilage and bones are particularly susceptible to aberrant mechanical transformation, which has long been associated with changes to the solid tissue components. There is, however, a growing appreciation for the role of interstitial fluid and tissue water content in degenerative mechanical transformation associated with orthopedic diseases. Yet, the contribution of viscous fluid-like behavior to the overall mechanical integrity of orthopedic tissues remains largely unexplored. We demonstrate wideband laser Speckle rHEologicAl micRoscopy (SHEAR) that harnesses speckle fluctuation induced by natural thermal motion of native light scattering tissue structures for microrheological investigation of orthopedic tissues.
Vagus nerve stimulation (VNS) yields promising therapeutic benefits but is often limited by off-target effects. Imaging tools that reveal the fascicular organization within the nerve can be used to better direct stimulation energy to the intended target. Here, we demonstrate polarization-sensitive (PS)-OCT imaging of an intact 7.5 cm ex-vivo porcine vagus nerve. The limited OCT penetration depth was overcome by using a dual-scanning microscope configured to simultaneously acquire B-scans from the upper and lower segments of a nerve sample. Three-dimensional maps of the complex fascicular organization in these nerves were generated.
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