We report for the first time in situ observations of a relatively rare secondary iron arsenate-sulphate mineral named bukovskýite – Fe3+2(As5+O4)(S6+O4)(OH)•7(H2O) - found in a shock melt vein of the Tissint Martian meteorite. It is hypothesised that the mineral formed when high concentrations of aqueous H+, Fe(III), SO4 and AsO4 were maintained for long periods of time in microenvironments created within wet subsurface Martian clays. The aqueous H+, Fe(III), SO4 and AsO4 species arose from the microbial oxidation of FeS2 with concurrent release of sequestrated As. The availability of aqueous AsO4 would also be complemented by dissolution by-products of the microbial reduction of Feoxides influenced by dissolved organic matter that alters the redox state and the complexation of As, thus shifting As partitioning in favour of the solute phase. This hypothesis is substantially supported by SEM analysis of a 15μm spherical structure comprising of a carbonaceous outer coating with a inner core of FeS2 (pyrite) that showed the pyrite surface with spherical pits, and chains of pits, with morphologies distinct from abiotic alteration features. The pits and channels have a clustered, geometric distribution, typical of microbial activity, and are closely comparable to biologically mediated microstructures created by Fe- and S-oxidising microbes in the laboratory. These microstructures are interpreted as trace fossils resulting from the attachment of bacteria to the pyrite surfaces.
Earlier studies of the Tissint Martian meteorite identified the presence of a number of 5-50μm carbonaceous spherical structures. SEM and EDS elemental spectra for 11 selected structures confirmed that they comprise of a carbonaceous outer coating with a inner core of FeS2 (pyrite) and are characterised as immiscible globules with curved boundaries. Here we report on the results of Raman spectroscopic studies that unambiguously confirm the mantle as comprising of ‘disordered carbonaceous material’. R1 = ID/IG against ΓD (cm-1) band parameter plots of the carbonaceous coatings imply a complex precursor carbon inventory comparable to the precursor carbon component of materials of known biotic source (plants, algae, fungi, crustaceans, prokaryotes). Correlation between peak metamorphic temperatures and Raman D-band (ΓD) parameters further indicate the carbonaceous component was subjected to a peak temperature of ~250 OC suggesting a possible link with the hydrothermal precipitation processes responsible for the formation of similar globules observed in hydrothermal calcite veins in central Ireland. Ω G (cm-1), ΓG (cm-1), Ω D (cm-1) and ΓD (cm-1) parameters further imply a level of crystallinity and disorder of the carbon component consistent with carbonaceous material recovered from a variety of non-terrestrial sources. Cl, N, O and S to C elemental ratios are typical of high volatility bituminous coals and distinctly higher than equivalent graphite standards.
We report for the first time in situ observations of 5-50μm spherical carbonaceous structures in the Tissint Martian meteorite comprising of pyrite (FeS2) cores and carbonaceous outer coatings. The structures are characterized as smooth immiscible spheres with curved boundaries occasionally following the contours of the pyrite inclusion. The structures bear striking resemblance to similar-sized immiscible carbonaceous spheres found in hydrothermal calcite vein deposits in the Mullaghwornia Quarry in central Ireland. Similar structures have been reported in Proterozoic and Ordovician sandstones from Canada as well as in a variety of astronomical sources including carbonaceous chondrites, chondritic IDPs and primitive chondritic meteorites. SEM and X-Ray elemental mapping confirmed the presence of organic carbon filling the crack and cleavage space in the pyroxene substrate, with further evidence of pyrite acting as an attractive substrate for the collection of organic matter. The detection of precipitated carbon collecting around pyrite grains is at variance with an igneous origin as proposed for the reduced organic component in Tissint, and is more consistent with a biogenetic origin.
The question of the contamination of meteorites by modern environmental microorganisms is an issue that has been raised since evidence for biological remains in carbonaceous meteorites was first published in the early 1960's.1-3 The contamination hypothesis has been raised for recent fossils of diatoms and filamentous cyanobacteria found embedded in the stones even though the nitrogen content of the fossils was below the 0.5% detection limit for Energy Dispersive X-ray Spectroscopy (EDS) of the Field Emission Scanning Electron Microscope. All modern biological contaminants should have nitrogen content in the detectable range of 2% to 20% indicating the remains are ancient fossils rather than living or Holocene cells. In our work, the possibility that extremophilic bacteria from our lab collection might be able to metabolize organic matter in the studied meteorites was tested. The potential toxic or inhibitory growth effects were also checked for different anaerobic cultures. UV exposed meteorite samples with consequent sterile extraction of the internal part were subjected to anaerobic cultivation techniques. As a result, eight anaerobic strains were isolated from internal and exterior parts of the studied meteorites. Preliminary results of their morphology, cytology, physiology, and molecular (16SrRNA sequencing) studies are presented and discussed in this article.
We report on the physical, chemical and mineral properties of a series of stone fragments recovered from the North Central Province of Sri Lanka following a witnessed fireball event on 29 December 2012. The stones exhibit highly porous poikilitic textures comprising of isotropic silica-rich/plagioclase-like hosts. Inclusions range in size and shape from mm-sized to smaller subangular grains frequently more fractured than the surrounding host and include ilmenite, olivine (fayalitic), quartz and accessory zircon. Bulk mineral compositions include accessory cristobalite, hercynite, anorthite, wuestite, albite, anorthoclase and the high pressure olivine polymorph wadsleyite, suggesting previous endurance of a shock pressure of ~20 GPa. Further evidence of shock is confirmed by the conversion of all plagioclase to maskelynite. Here the infrared absorption spectra in the region 580 cm-1 to 380 cm-1 due to the Si-O-Si or Si-O-Al absorption band shows a partial shift in the peak at 380 cm-1 towards 480 cm-1 indicating an intermediate position between crystalline and amorphous phase. Host matrix chemical compositions vary between samples, but all are rich in SiO2. Silica-rich melts display a heterogeneous K-enrichment comparable to that reported in a range of non-terrestrial material from rare iron meteorites to LL chondritic breccias and Lunar granites. Bulk chemical compositions of plagioclase-like samples are comparable to reported data e.g. Miller Ranger 05035 (Lunar), while Si-rich samples accord well with mafic and felsic glasses reported in NWA 1664 (Howardite) as well as data for fusion crust present in a variety of meteoritic samples. Triple oxygen isotope results show Δ17O = -0.335 with δ18O (‰ rel. SMOW) values of 17.816 ± 0.100 and compare well with those of known CI chondrites and are within the range of CI-like (Meta-C) chondrites. Rare earth elemental abundances show a profound Europium anomaly of between 0.7 and 0.9 ppm while CI normalized REE patterns accord well with those of high potassium and high aluminium glasses found in lunar and 4 Vesta samples. Twoelement discrimination maps of FeO vs SiO2, FeO vs TiO2, FeO vs Al2O3 and FeO vs Na2O similarly match those of impact glasses present in lunar samples and remain within relatively close proximity of the KREEP component. Iridium levels of between 1-7ppm, approximately 104 times that of terrestrial crustal rocks, were detected in all samples.
On December 29, 2012, a bright yellow and green fireball was observed to disintegrate over the Polonnaruwa District of North Central, Sri Lanka. Many low density, black stones were recovered soon after the observed fall from rice paddy fields near the villages of Aralaganwila and Dimbulagala. These stones were initially studied by optical microscopy methods at the Medical Research Institute in Colombo, Sri Lanka. Soon thereafter, samples were sent to the UK and to the United States. More extensive Field Emission Scanning Electron Microscopy studies were then carried out at Cardiff University and the NASA/Marshall Space Flight Center. The physico-chemical properties, elemental abundances, mineralogy and stable isotope data clearly indicate that these stones are non-terrestrial. Freshly fractured interior surfaces of the black stones have also been observed to contain the remains of fossilized diatom. Many of the diatom frustules are clearly embedded in the meteorite rock matrix and exhibit nitrogen levels below the EDX detection limits. Some of the fossil diatoms are araphid marine pennates and planktonic forms that are inconsistent with conditions associated with rice paddy fields. These observations indicate the fossilized diatoms are indigenous to the meteorites rather than post-arrival biological contaminants. The carbon content and mineralogy suggests that these stones may represent a previously ungrouped clan of carbonaceous meteorites. The extremely low density (~0.6) of the stones and their observed mineralogy was inconsistent with known terrestrial rocks (e.g., pumice, diatomite and fulgurites). The minerals detected suggest that the parent body of the Polonnaruwa stones may have been the nucleus of a comet. These observations are interpreted as supporting the Hoyle-Wickramasinghe Panspermia hypothesis and the hypothesis that diatoms and other microorganisms might be capable of living and growing in water ice and brines in comets.
Recent studies of comets and cometary dust have confirmed the presence of biologically relevant organic molecules
along with clay minerals and water ice. It is also now well established by deuterium/hydrogen ratios that the CI1
carbonaceous meteorites contain indigenous extraterrestrial water. The evidence of extensive aqueous alteration of the
minerals in these meteorites led to the hypothesis that water-bearing asteroids or comets represent the parent bodies of
the CI1 (and perhaps CM2) carbonaceous meteorites. These meteorites have also been shown to possess a diverse array
of complex organics and chiral and morphological biomarkers. Stable isotope studies by numerous independent
investigators have conclusively established that the complex organics found in these meteorites are both indigenous and
extraterrestrial in nature. Although the origin of these organics is still unknown, some researchers have suggested that
they originated by unknown abiotic mechanisms and may have played a role in the delivery of chiral biomolecules and
the origin of life on Early Earth.
Preliminary SEM/EDAX studies of the Tissint meteorite shows projections of interior spherical globules rich in C and O. Such concentrations of carbonaceous material in a matrix of mineral grains pose a mystery. These structures are consistent with remnants of biological structures.
Recent studies of comets and cometary dust have confirmed the presence of biologically relevant organic molecules
along with clay minerals and water ice. It is also now well established by deuterium/hydrogen ratios that the CI1
carbonaceous meteorites contain indigenous extraterrestrial water. The evidence of extensive aqueous alteration of the
minerals in these meteorites led to the hypothesis that water-bearing asteroids or comets represent the parent bodies of
the CI1 (and perhaps CM2) carbonaceous meteorites. These meteorites have also been shown to possess a diverse array
of complex organics and chiral and morphological biomarkers. Stable isotope studies by numerous independent
investigators have conclusively established that the complex organics found in these meteorites are both indigenous and
extraterrestrial in nature. Although the origin of these organics is still unknown, some researchers have suggested that
they originated by unknown abiotic mechanisms and may have played a role in the delivery of chiral biomolecules and
the origin of life on Early Earth.
In this paper we review these results and investigate the thermal history of comets. We show that permanent as well as
transient domains of liquid water can be maintained on a comet under a plausible set of assumptions. With each
perihelion passage of a comet volatiles are preferentially released, and during millions of such passages the comet could
shed crustal debris that may survive transit through the Earth's atmosphere as a carbonaceous meteorite. We review the
current state of knowledge of comets and carbonaceous meteorites. We also present the results of recent studies on the
long-term viability of terrestrial ice-microbiota encased in ancient glacial ice and permafrost. We suggest that the
conditions which have been observed to prevail on many comets do not preclude either survivability (or even the active
metabolism and growth) of many types of eukaryotic and prokaryotic microbial extremophiles-including algae,
cyanobacteria, bacteria and archaea. It is argued that the chemical and morphological biomarkers detected on comets and
carbonaceous meteorites can be explained by ancient microbial activity without the need to invoke unknown abiotic
production mechanisms.
Three decades ago the first convincing evidence of microbial fossils in carbonaceous chondrites was discovered and
reported by Hans Dieter Pflug and his collaborators. In addition to morphology, other data, notably laser mass
spectroscopy, confirmed the identification of such structures as putative bacterial fossils. Balloon-borne cryosampling
of the stratosphere enables recovery of fragile cometary dust aggregates with their structure and carbonaceous matter
largely intact. SEM studies of texture and morphology of particles in the Cardiff collection, together with EDX
identifications, show two main types of putative bio-fossils - firstly organic-walled hollow spheres around 10μm across,
secondly siliceous diatom skeletons similar to those found in carbonaceous chondrites and terrestrial sedimentary rocks
and termed 'acritarchs'. Since carbonaceous chondrites (particularly Type 1 chondrites) are thought to be extinct
comets the data reviewed in this article provide strong support for theories of cometary panspermia.
We explore the conditions prevailing in primordial planets in the framework of the HGD cosmologies as discussed by
Gibson and Schild. The initial stages of condensation of planet-mass gas clouds is set at 300,000 yr (0.3My) following
the onset of plasma instabilities when ambient temperatures were >1000K. Eventual collapse of the cloud into a solid
structure, dominated by water-ice and organics takes place against the background of an expanding universe with
declining ambient temperatures. Isothermal free fall collapse occurs initially via quasi equilibrium polytropes until
opacity sets in due to molecule and dust formation. The contracting cooling cloud is a venue for molecule formation and
the sequential condensation of solid particles, starting from mineral grains at high temperatures to ice particles at lower
temperatures, Water-ice becomes thermodynamically stable between 7 and 15 My after the initial onset of collapse, and
contraction to form a solid icy core begins shortly thereafter. The icy planet core, which includes a fraction of
radioactive nuclides, 26Al and 60Fe, melts through interior heating. We show, using heat conduction calculations, that the
interior domains remain liquid for tens of My for 300km and 1000km objects, but not for 30 or 50km objects. Initially
planets are separated by relatively short distances, measured in tens to hundreds of AU, because of the high density of
the early universe. Thus exchanges of materials, organic molecules and evolving templates could readily occur
providing optimal conditions for an initial origin of life. The condensation of solid molecular hydrogen as an extended
outer crust takes place much later in the collapse history of the protoplanet. When the object has shrunk to several times
the radius of Jupiter, the hydrogen partial pressure exceeds the saturation vapour pressure of solid hydrogen at the
ambient temperature and condensation occurs.
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